The phospholipases responsible for hydrolysis of glycerophospholipids are divided into two groups: acylhydrolases and phosphodiesterases. Ghorani V, Marefati N, Shakeri F, Rezaee R, Boskabady M, Boskabady MH. Importance of group X-secreted phospholipase A2 in allergen-induced airway inflammation and remodeling in a mouse asthma model.

… Function of secreted phospholipase A2 group-X in asthma and allergic disease. Elevated secreted phospholipase A 2 (sPLA 2) activity in the airways has been implicated in the pathogenesis of asthma and allergic disease for some time.The identity and function of these enzymes in asthma is becoming clear from work in our lab and others. Asthma is a chronic inflammatory lung disease with considerable unmet medical needs for new and effective therapies. View Article Google Scholar 8. 15 Citations (Scopus) Abstract. Bee venom phospholipase A2 suppresses allergic airway inflammation in an ovalbumin‐induced asthma model through the induction of regulatory T cells Soojin Park Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki‐Dong, Dongdaemoon‐Gu, Seoul, 130‐701 Republic of … Schaloske RH, Dennis EA (2006) The phospholipase A2 superfamily and its group numbering … The …

This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond, releasing arachidonic acid and lysophosphatidic acid. The increased morbidity, mortality, and ineffective treatment associated with the pathogenesis of chronic inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD) have generated much research interest. The main substrates for these enzymes are glycerophospholipids which contain glycerol with a saturated fatty acid in the sn-1 position and an unsaturated fatty acid in the sn-2 position. Some cell samples were preincubated in presence of phosphatidylserine for 30 min, then concanavalin A (Con A) was added for further 15 min. Background: Secretory phospholipases A2 (sPLA2) have functions relevant to asthmatic inflammation, including eicosanoid synthesis and effects on dendritic cells and T cells. Because elevated lipoprotein-associated phospholipase A2 activity is positively associated with coronary heart disease (Lp-PLA2 Studies Collaboration, 2010), Polfus et al. High activity of group V phospholipases A2 to induce eicosanoid biosynthesis in neighboring inflammatory cells. Phospholipase A2 Activity Test GENERAL. J Exp Med 2007 ;204: 865 – 877 .

Iran J Allergy Asthma Immunol. Although sPLA 2 s function as enzymes, some of the sPLA 2 s bind with high affinity to a C-type lectin receptor, called PLA2R1, which has functions in both cellular signaling and clearance of sPLA 2 s.

The Effects of Allium Cepa Extract on Tracheal Responsiveness, Lung Inflammatory Cells and Phospholipase A2 Level in Asthmatic Rats. J Biol Chem 281: 10935–10944. The secreted PLA2s and the lipoprotein-associated phospholipase A2 (Lp-PLA2) have been associated with atherogenesis and its complications. (2015) sequenced the exomes of 6,325 participants in the ARIC (Atherosclerosis Risk in Communities) study to find genetic variants that lowered phospholipase A2 activity. Author information: (1)Department of Medicine, Division of Pulmonary, Critical Care and Sleep, University of Washington, Seattle, WA, United States of America. Phospholipases A2 (PLA2s) EC 3.1.1.4 are enzymes that cleave fatty acid in position two of phospholipids, hydrolyzing the bond between the second fatty acid “tail” and the glycerol molecule. Phospholipase C and phospholipase A2 inhibitors decreased vancomycin-induced histamine release, but not calcium ionophore A23187-induced release. Secreted phospholipase A 2 s (sPLA 2 s) regulate eicosanoid formation and have been implicated in asthma. Cytosolic phospholipase A2α (cPLA2α) is the rate-limiting enzyme that is ultimately responsible for the production of eicosanoids implicated in the pathogenesis of asthma.

Neil Misso, N. Petrovic, C. Grove, Tony Celenza, J. Brooks-Wildhaber, Philip Thompson.