It has low acid sensitivity. The dynamics and electronic absorption spectrum of enhanced cyan fluorescent protein (ECFP), a mutant of green fluorescent protein (GFP), have been studied by means of a 1 ns molecular dynamics (MD) simulation. The use of fluorescent proteins for in vivo imaging has opened many new areas of research . However, yellow fluorescent protein presents some problems in that it is very sensitive to acidic pH and loses approximately 50 … 1 Fluorescent Fusion Proteins. Cyan fluorescent protein Imported Organism i: Acropora aculeus Imported. Among the important advances in the field have been the development of transgenic mice expressing various fluorescent proteins including green fluorescent protein (GFP) , red fluorescent protein (RFP) , and cyan fluorescent protein (CFP) . Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. Visualize and measure gene expression in a variety of organisms from bacteria to higher plants and animals with our selection of cyan and green fluorescent proteins. Cyan and green fluorescent proteins. CFP, Cyan Fluorescent Protein: Source: E. coli: Appearance: Lyophilized protein: Physical Form Description: Freeze Dried: Molecular Weight: 31.3 kDa: Purity by SDS-PAGE ≥97%: Purity by ≥97%: Endotoxin Level: 0.1 ng/μg: Reconstitution Instructions: Reconstitute with dH₂O to 1 mg/ml: Handling: Centrifuge the vial prior to opening. The two X-ray conformations A‘ and B‘ of ECFP were considered. The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. Use of these fluorescent proteins can be limited by their thermosensitivity, dim fluorescence, and tendency for aggregation. Biochem J. Cyan Fluorescent Protein. 400(3): p. 531-540. Ai HW, et al., Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications.

Organism i: Micromussa lordhowensis Imported. Enhanced cyan and yellow fluorescent proteins are widely used for dual color imaging and protein−protein interaction studies based on fluorescence resonance energy transfer. In addition to wild-type GFP and GFPuv proteins, we offer monomeric AcGFP1 , which is ideal for performing fusion studies. Hanna Fares, Alexander M van der Bliek, in Methods in Cell Biology, 2012.

Here we report the results of a site-directed mutagenesis approach to improve these fluorescent … The chromophore was assumed to be neutral, and all titratable residues were taken in their standard … 2006. Enhanced yellow fluorescent protein is also useful for energy transfer experiments when paired with enhanced cyan fluorescent protein (ECFP) or GFP2.